EBTT 2005
Archived edition

EBTT 2005

Electroporation based Technologies and Treatments — International Scientific Workshop & Postgraduate Course

14–20 November 2005Ljubljana, Slovenia15 ECTS

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

Whether you are a postgraduate student or you just want to learn and contribute to the discussion, you were kindly invited to attend the workshop and the course on electroporation technologies and treatments. Abstract submission was strongly encouraged and was necessary if credits (15 ECTS) were sought.

Organised by the University of Ljubljana, Faculty of Electrical Engineering, and the Institute of Oncology, Ljubljana.

Organising committee

Chair: Peter Kramar
Members: Maša Kandušer, Gorazd Pucihar, Blaž Valič, Selma Čorović, Mojca Pavlin, Nataša Pavšelj, Matej Reberšek, Anže Županič

Course content

Key lectures & topics

Cells and tissues in electric fields

D. Miklavčič — biological cell & plasma membrane; resting & induced transmembrane voltage; static & dynamic fields; temperature & conductivity changes.

Physical chemistry of membrane electroporation

E. Neumann — electrochemical membrane thermodynamics; electroporative transport.

Electroporation in vitro

J. Teissié — cells in suspension and in 2D tissue models; parameters governing efficiency in vitro.

Electroporation in vivo

L. M. Mir — electroporation of cells in tissue; methods for detecting cell electropermeabilization in vivo.

Development of devices and electrodes

D. Miklavčič — devices for in vitro, in vivo and clinical purposes; electrodes.

Electrochemotherapy of tumours

G. Serša — experiments in vitro & in vivo; current clinical applications; future perspectives.

Gene transfection

L. M. Mir & J. Teissié — gene transfection in vitro/in vivo; gene expression regulation by siRNA electrotransfer.

Electroporation in biotechnology

J. Teissié — water sterilization and other applications; future perspectives.

Transdermal drug delivery

V. Préat — transdermal delivery using electroporation; intradermal & topical delivery of DNA.

Students & participants presentations

Short presentations by students and participants.

Additional topics

Faculty

Co-directors, faculty & invited lecturers

Co-DirectorDamijan Miklavčič
Co-DirectorLluis M. Mir

Faculty members & lecturers

  • Damijan MiklavčičUniversity of Ljubljana, Slovenia
  • Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
  • Eberhard NeumannUniversity of Bielefeld, Germany
  • Véronique PréatCatholic University of Louvain, Brussels, Belgium
  • Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
  • Justin TeissiéCNRS & IPBS, Toulouse, France

Invited lecturers

  • Bettina CoudercInstitut Claudius Regaud, Toulouse, France
  • Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
  • Muriel GolzioCNRS & IPBS, Toulouse, France
  • Sergej KakorinUniversity of Bielefeld, Germany
  • Juergen F. KolbOld Dominion University, Norfolk, USA
  • Tadej KotnikUniversity of Ljubljana, Slovenia
  • Igor LackovićUniversity of Zagreb, Croatia
  • Alenka Maček LebarUniversity of Ljubljana, Slovenia
  • Marie-Pierre RolsCNRS & IPBS, Toulouse, France
  • Zvonimir RudolfInstitute of Oncology, Ljubljana, Slovenia
  • Matjaž ZwitterInstitute of Oncology, Ljubljana, Slovenia

Programme & schedule

Day-by-day schedule

Six-day programme, 14–20 November 2005. Download the full schedule (PDF)

Day 1 — Monday 14 November

8:30Opening lecture
Damijan Miklavčič
9:15Cells in electric fields
Tadej Kotnik
10:00Coffee break
10:30Tissue in electric fields
Igor Lacković
11:15Electrothermodynamics analysis of Membrane Electroporation (1/2)
Eberhard Neumann
12:00Lunch break
13:30Invited lecture — Effects of ultra short electric pulses on cells
Juergen F. Kolb
15:00Coffee break
15:30Students' presentations
16:15Practical work and discussion groups (1)

Day 2 — Tuesday 15 November

8:30Electrothermodynamics analysis of Membrane Electroporation (2/2)
Eberhard Neumann
9:15Kinetic Analysis of electroporative Transport
Sergej Kakorin
10:00Coffee break
10:30Electroporation in vitro
Justin Teissié
12:00Lunch break
13:30Invited lecture — Numerical modelling techniques in biotechnology
Alenka Maček Lebar
14:15Invited lecture — Tumor biology
Maja Čemažar
15:00Coffee break
15:30Students' presentations
16:15Practical work and discussion groups (2)

Day 3 — Wednesday 16 November

8:30Electroporation in vivo
Lluis M. Mir
10:00Coffee break
10:30Development of devices and electrodes
Damijan Miklavčič
12:00Lunch break
13:30Invited lecture — Transduction, transcription, regulation and gene expression
Bettina Couderc
15:00Coffee break
15:30Students' presentations
16:15Practical work and discussion groups (3)

Day 4 — Thursday 17 November

8:30Gene transfection in vitro
Marie-Pierre Rols
9:15Gene transfection in vivo
Lluis M. Mir
10:00Coffee break
10:30Gene expression regulation by siRNA electrotransfer
Muriel Golzio
11:15Lunch break
12:00Social event — Trip to Postojna cave

Day 5 — Friday 18 November

8:30Application of electroporation in electrochemotherapy of tumours
Gregor Serša
10:00Coffee break
10:30Clinical electrochemotherapy
Zvonimir Rudolf
11:15Skin and transdermal drug delivery
Véronique Préat
12:00Lunch break
13:30Invited lecture — Ethics in biomedical research
Matjaž Zwitter
15:00Coffee break
15:30Students' presentations
16:15Practical work and discussion groups (4)

Day 6 — Saturday 19 November

8:30Electroporation in transdermal drug delivery
Véronique Préat
9:15Electroporation in skin gene delivery
Véronique Préat
10:00Coffee break
10:30Application of electroporation in biotechnology
Justin Teissié · Closing lecture Lluis M. Mir
12:00Lunch break
13:30Exams
15:00Coffee break
15:30Certificates and goodbye

Registration

Tuition fee & deadlines

The tuition fee (580 EUR) included attendance to the lectures, book of abstracts and course material, lunches and social events, certificate of attendance and examination (ECTS credits). A limited number of grants for student accommodation was available on request.

Important deadlines (archived):
Registration & abstract submission: 1 September 2005.
Payment of the tuition fee: 10 October 2005.

Forms

Abstracts & proceedings

Abstract submission & proceedings

Download the abstract submission form and instructions for authors. For abstract writing, the enclosed MS Word template was used.

EBTT 2005 proceedings cover

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Getting here & staying

Venue & accommodation

Held at the Faculty of Electrical Engineering, University of Ljubljana. Map of Ljubljana centre

Support

Sponsors & partners

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros
info@albatros-bled.com
tel +386 4 5780 350 · fax +386 4 5780 355

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