Electroporation based Technologies and Treatments — International Scientific Workshop & Postgraduate Course
The meeting
Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.
Whether you are a postgraduate student or you just want to learn and contribute to the discussion, you were kindly invited to attend the workshop and the course on electroporation technologies and treatments. Abstract submission was strongly encouraged and was necessary if credits (15 ECTS) were sought.
Organised by the University of Ljubljana, Faculty of Electrical Engineering, and the Institute of Oncology, Ljubljana.
Chair: Peter Kramar
Members: Maša Kandušer, Gorazd Pucihar, Blaž Valič, Selma Čorović, Mojca Pavlin, Nataša Pavšelj, Matej Reberšek, Anže Županič
Course content
D. Miklavčič — biological cell & plasma membrane; resting & induced transmembrane voltage; static & dynamic fields; temperature & conductivity changes.
E. Neumann — electrochemical membrane thermodynamics; electroporative transport.
J. Teissié — cells in suspension and in 2D tissue models; parameters governing efficiency in vitro.
L. M. Mir — electroporation of cells in tissue; methods for detecting cell electropermeabilization in vivo.
D. Miklavčič — devices for in vitro, in vivo and clinical purposes; electrodes.
G. Serša — experiments in vitro & in vivo; current clinical applications; future perspectives.
L. M. Mir & J. Teissié — gene transfection in vitro/in vivo; gene expression regulation by siRNA electrotransfer.
J. Teissié — water sterilization and other applications; future perspectives.
V. Préat — transdermal delivery using electroporation; intradermal & topical delivery of DNA.
Short presentations by students and participants.
Faculty
Programme & schedule
Six-day programme, 14–20 November 2005. Download the full schedule (PDF)
| 8:30 | Opening lecture Damijan Miklavčič |
| 9:15 | Cells in electric fields Tadej Kotnik |
| 10:00 | Coffee break |
| 10:30 | Tissue in electric fields Igor Lacković |
| 11:15 | Electrothermodynamics analysis of Membrane Electroporation (1/2) Eberhard Neumann |
| 12:00 | Lunch break |
| 13:30 | Invited lecture — Effects of ultra short electric pulses on cells Juergen F. Kolb |
| 15:00 | Coffee break |
| 15:30 | Students' presentations |
| 16:15 | Practical work and discussion groups (1) |
| 8:30 | Electrothermodynamics analysis of Membrane Electroporation (2/2) Eberhard Neumann |
| 9:15 | Kinetic Analysis of electroporative Transport Sergej Kakorin |
| 10:00 | Coffee break |
| 10:30 | Electroporation in vitro Justin Teissié |
| 12:00 | Lunch break |
| 13:30 | Invited lecture — Numerical modelling techniques in biotechnology Alenka Maček Lebar |
| 14:15 | Invited lecture — Tumor biology Maja Čemažar |
| 15:00 | Coffee break |
| 15:30 | Students' presentations |
| 16:15 | Practical work and discussion groups (2) |
| 8:30 | Electroporation in vivo Lluis M. Mir |
| 10:00 | Coffee break |
| 10:30 | Development of devices and electrodes Damijan Miklavčič |
| 12:00 | Lunch break |
| 13:30 | Invited lecture — Transduction, transcription, regulation and gene expression Bettina Couderc |
| 15:00 | Coffee break |
| 15:30 | Students' presentations |
| 16:15 | Practical work and discussion groups (3) |
| 8:30 | Gene transfection in vitro Marie-Pierre Rols |
| 9:15 | Gene transfection in vivo Lluis M. Mir |
| 10:00 | Coffee break |
| 10:30 | Gene expression regulation by siRNA electrotransfer Muriel Golzio |
| 11:15 | Lunch break |
| 12:00 | Social event — Trip to Postojna cave |
| 8:30 | Application of electroporation in electrochemotherapy of tumours Gregor Serša |
| 10:00 | Coffee break |
| 10:30 | Clinical electrochemotherapy Zvonimir Rudolf |
| 11:15 | Skin and transdermal drug delivery Véronique Préat |
| 12:00 | Lunch break |
| 13:30 | Invited lecture — Ethics in biomedical research Matjaž Zwitter |
| 15:00 | Coffee break |
| 15:30 | Students' presentations |
| 16:15 | Practical work and discussion groups (4) |
| 8:30 | Electroporation in transdermal drug delivery Véronique Préat |
| 9:15 | Electroporation in skin gene delivery Véronique Préat |
| 10:00 | Coffee break |
| 10:30 | Application of electroporation in biotechnology Justin Teissié · Closing lecture Lluis M. Mir |
| 12:00 | Lunch break |
| 13:30 | Exams |
| 15:00 | Coffee break |
| 15:30 | Certificates and goodbye |
Registration
The tuition fee (580 EUR) included attendance to the lectures, book of abstracts and course material, lunches and social events, certificate of attendance and examination (ECTS credits). A limited number of grants for student accommodation was available on request.
Important deadlines (archived):
Registration & abstract submission: 1 September 2005.
Payment of the tuition fee: 10 October 2005.
Abstracts & proceedings
Download the abstract submission form and instructions for authors. For abstract writing, the enclosed MS Word template was used.
Electronic proceedings — all abstracts and course material.
Download proceedings (PDF)Getting here & staying
Held at the Faculty of Electrical Engineering, University of Ljubljana. Map of Ljubljana centre
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